Cellular resolution multiplexed FLIM tomography with dual-color Bessel beam.
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Cellular resolution multiplexed FLIM tomography with dual-color Bessel beam.
Fourier flim multiplexing (FmFLIM) allows multiplexing tomography 3D imaging lifetime of the entire embryo. In FmFLIM our previous system, the spatial resolution is limited to 25 pM as a trade-off between spatial resolution and imaging depth. In order to achieve cellular resolution imaging of thick specimens, we build tomography system with dual-color Bessel beams.
In combination with FmFLIM, Bessel FmFLIM tomography system can perform parallel lifetime imaging 3D on channel excitation-emission couple on cellular resolution of 2.8 um. Ability Bessel tomographic image of the system is shown by 3D imaging FmFLIM lifetime of dual-labeled transgenic zebrafish embryos.
Quantum-dot nanoprobes and AOTF based cross talk is eliminated six color imaging of biomolecules in cellular systems.
Primary cell cultures mimic the physiology and genetics of in-vivo tissue of origin, fixed, complications in the derivation and propagation of primary cell cultures restrict their use in biological research. However, in-vitro model using primary cells may be complementary models mimic in vivo responses.
However, conventional techniques such as western blot and PCR were used to study the expression and activation of protein requires a large number of cells, then repeated formation and maintenance of primary cultures that can not be avoided. Quantum dots (Q-dots) and acousto-optic tunable filter (AOTF) based multiplex imaging system is an alternative option for evaluating some signaling molecules by using a small number of cells.
Q-Point has a broad excitation and narrow emission spectrum, which makes it possible to simultaneously excite several Q-points using a single excitation wavelength. The use of AOTF in fluorescence detection system makes it possible to scan the fluorescence emission intensity of the Q-dot in their center wavelengths, this phenomenon effectively avoid the spectral overlap between neighboring Q-dots.
Cellular resolution multiplexed FLIM tomography with dual-color Bessel beam.
When the Q-point conjugated with antibodies act as an effective sensing probe. To validate this, the pattern of expression of p-JNK-1, p-GSK3β, p-IRS1ser, p-IRS1tyr, p-FOXO1, and PPAR-γ, which is involved in insulin resistance are simultaneously monitored in adipocytes and HepG2 cells co culture model , Results observed clearly show that PPAR-γ is an important component in the development of insulin resistance.
Description: Please reffer to the technical data sheet for more detail information for this item. Our dedicated team would be happy to assist you via live chat, email or phone.
Description: Please reffer to the technical data sheet for more detail information for this item. Our dedicated team would be happy to assist you via live chat, email or phone.
Description: Please reffer to the technical data sheet for more detail information for this item. Our dedicated team would be happy to assist you via live chat, email or phone.
Description: Please reffer to the technical data sheet for more detail information for this item. Our dedicated team would be happy to assist you via live chat, email or phone.
Cas9 Protein and T7 gRNA SmartNuclease Synthesis Kit
Description: Recombinant Streptomyces Avidinii Streptavidin produced in E.Coli is a single, non-glycosylated polypeptide chain (25-183) containing a total of 167 amino acids and having a molecular mass of 17kDa. The Streptavidin protein is fused to an 8 aa N-terminal His-Tag and purified by proprietary chromatographic techniques.
Buffer: Preservative: 0.03% Proclin 300 Constituents: 50% Glycerol, 0.01M PBS, PH 7.4 >95%, Protein G purified
Description: A polyclonal antibody against Streptavidin. Recognizes Streptavidin from Streptomyces avidinii. This antibody is Unconjugated. Tested in the following application: ELISA
Buffer: Preservative: 0.03% Proclin 300 Constituents: 50% Glycerol, 0.01M PBS, PH 7.4 >95%, Protein G purified
Description: A polyclonal antibody against Streptavidin. Recognizes Streptavidin from Streptomyces avidinii. This antibody is Unconjugated. Tested in the following application: ELISA
Description: Streptavidin is a protein produced by Streptomyces avidinii and isolated by purification from fermentation broth. The pure, homogeneous protein shows predominantly one single band in SDS PAGE. Streptavidin consists of 4 identical subunits, each bearing an active binding site for biotin. Streptavidin has a molecular weight of 55kDa.
Description: Please reffer to the technical data sheet for more detail information for this item. Our dedicated team would be happy to assist you via live chat, email or phone.
Description: Please reffer to the technical data sheet for more detail information for this item. Our dedicated team would be happy to assist you via live chat, email or phone.
Description: Please reffer to the technical data sheet for more detail information for this item. Our dedicated team would be happy to assist you via live chat, email or phone.
Description: Please reffer to the technical data sheet for more detail information for this item. Our dedicated team would be happy to assist you via live chat, email or phone.
AAVS1 Safe Harbor Targeting Vector 2.0 - All-Purpose Donor (AAVS1-SA-puro-MCS), Complete Kit with CAS601A-1 (Cas9 SmartNuclease AAVS1-gRNA Targeting Vector) and GE640PR-1 (Junction PCR Primer Mix to confirm AAVS1 integration site)
Description: Minimum order quantity: 1 unit of 1mg
In addition, the results prove that the Q-dot based imaging methodologies developed AOTF is a reasonable option to simultaneously monitor multiple cell signaling molecules with a limited population.
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-T2A-Puro SmartNickase Lentivector Plasmid + LentiStarter Packaging Kit)
-EF1-GFP SmartNickase Lentivector Plasmid + LentiStarter Packaging Kit)
-T2A-Puro SmartNickase Lentivector Plasmid + LentiStarter Packaging Kit)
-EF1-GFP SmartNickase Lentivector Plasmid + LentiStarter Packaging Kit)
 and Fast ligase (2.5ul)])
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, Complete Kit with CAS601A-1 (Cas9 SmartNuclease AAVS1-gRNA Targeting Vector) and GE640PR-1 (Junction PCR Primer Mix to confirm AAVS1 integration site))
, Complete Kit with CAS601A-1 (Cas9 SmartNuclease AAVS1-gRNA Targeting Vector) and GE640PR-1 (Junction PCR Primer Mix to confirm AAVS1 integration site))
, Complete Kit with CAS601A-1 (Cas9 SmartNuclease AAVS1-gRNA Targeting Vector) and GE640PR-1 (Junction PCR Primer Mix to confirm AAVS1 integration site))
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