An Analysis of the Practicalities of Multi-Color Nanoparticle Cellular Bar-Coding.

Many applications in biomedical research requires long-range identification and tracking of cells over time. In previous work we have shown that with sequential doses of a population of cells with nanoparticles of different emission wavelengths is possible to use a random number of nanoparticles loaded vesicles produced by cells as a barcode for individual cells in the population.

In this paper we develop a simple model to describe the amount of code that can be generated by using this sequential loading protocol. This methodology is validated in comparison with experiment and then used to predict the effects of various number of colors used to encode the cell and also to assess the effects of misread codes due to errors in the imaging cellular vesicles.

A Quick, Easy and Customizable Eight-color flow cytometric Method for Mobile Content Analysis of Bronchoalveolar Lavage Fluids in Mouse.

An Analysis of the Practicalities of Multi-Color Nanoparticle Cellular Bar-Coding.
An Analysis of the Practicalities of Multi-Color Nanoparticle Cellular Bar-Coding.

Cell composition of bronchoalveolar lavage fluid (BAL) is an important indicator of airway inflammation. It is generally determined by cytocentrifuging leukocytes on a slide, then staining, identify, and count them as eosinophils, neutrophils, macrophages, or lymphocytes according to morphological criteria under a light microscope, where it is not always easy to distinguish macrophages from the lymphocytes.

We describe here a single-step, easy to use, and easy-to-customize 8-color flow cytometric method to perform differential cell count and compare it with the number of morphology on stained cytospins. This method identifies BAL cells by one step immunolabeling simultaneous procedure using antibodies to identify T cells, B cells, neutrophils, eosinophils, and macrophages.

morphological analysis of flow-sorted cell subsets are used to validate this protocol. An important advantage of flow cytometry basic protocol is the ability to customize by adding antibodies to study the expression of receptors on the cell surface of leukocytes and identify subclasses of inflammatory cells as needed. © 2017 by John Wiley & Sons, Inc.

The advantage of dual-color fluorescence-tracing glioma orthotopic implantation model: Detecting the location of the tumor, angiogenesis, cell fusion and the tumor microenvironment.

Various organs of the body have different microenvironments with diverse biological characteristics that can influence tumor growth in themselves. However, the mechanisms underlying the interaction between tumor cells and the host is not currently well understood.

In this study, glioma model is the implantation of orthotopic fluorescence-tracing dual-color developed, in which the C6 cells rat glioma is labeled with a fluorescent dye red CM-Dil and glioma cells SU3 man stably express the protein fluorescence red, were inoculated into the right caudate nucleus of GMOs female C57BL / 6 nude mice revealed enhanced green fluorescent protein.

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Dual-color tracing the whole body in vivo fluorescence imaging of xenografts done using live imaging system. transplanted tumor frozen section prepared for histological analysis, to detect the presence of tumor cells invade blood vessels and cell fusion. dual-color images were able to distinguish between tumor cells red and green host cells. Results of this research suggest that the dual-color fluorescence-tracing glioma orthotopic implantation model may be convenient to detect the location of tumors, angiogenesis, cell fusion, and the tumor microenvironment.