An Analysis of the Practicalities of Multi-Color Nanoparticle Cellular Bar-Coding.

Many applications in biomedical research requires long-range identification and tracking of cells over time. In previous work we have shown that with sequential doses of a population of cells with nanoparticles of different emission wavelengths is possible to use a random number of nanoparticles loaded vesicles produced by cells as a barcode for individual cells in the population.

In this paper we develop a simple model to describe the amount of code that can be generated by using this sequential loading protocol. This methodology is validated in comparison with experiment and then used to predict the effects of various number of colors used to encode the cell and also to assess the effects of misread codes due to errors in the imaging cellular vesicles.

A Quick, Easy and Customizable Eight-color flow cytometric Method for Mobile Content Analysis of Bronchoalveolar Lavage Fluids in Mouse.

An Analysis of the Practicalities of Multi-Color Nanoparticle Cellular Bar-Coding.
An Analysis of the Practicalities of Multi-Color Nanoparticle Cellular Bar-Coding.

Cell composition of bronchoalveolar lavage fluid (BAL) is an important indicator of airway inflammation. It is generally determined by cytocentrifuging leukocytes on a slide, then staining, identify, and count them as eosinophils, neutrophils, macrophages, or lymphocytes according to morphological criteria under a light microscope, where it is not always easy to distinguish macrophages from the lymphocytes.

We describe here a single-step, easy to use, and easy-to-customize 8-color flow cytometric method to perform differential cell count and compare it with the number of morphology on stained cytospins. This method identifies BAL cells by one step immunolabeling simultaneous procedure using antibodies to identify T cells, B cells, neutrophils, eosinophils, and macrophages.

morphological analysis of flow-sorted cell subsets are used to validate this protocol. An important advantage of flow cytometry basic protocol is the ability to customize by adding antibodies to study the expression of receptors on the cell surface of leukocytes and identify subclasses of inflammatory cells as needed. © 2017 by John Wiley & Sons, Inc.

The advantage of dual-color fluorescence-tracing glioma orthotopic implantation model: Detecting the location of the tumor, angiogenesis, cell fusion and the tumor microenvironment.

Various organs of the body have different microenvironments with diverse biological characteristics that can influence tumor growth in themselves. However, the mechanisms underlying the interaction between tumor cells and the host is not currently well understood.

In this study, glioma model is the implantation of orthotopic fluorescence-tracing dual-color developed, in which the C6 cells rat glioma is labeled with a fluorescent dye red CM-Dil and glioma cells SU3 man stably express the protein fluorescence red, were inoculated into the right caudate nucleus of GMOs female C57BL / 6 nude mice revealed enhanced green fluorescent protein.

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Description: HRP conjugation kit for all types of IgG

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EUR 920
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EUR 408

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HRP Antibody

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EUR 2635

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EUR 1368

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EUR 1114

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HRP-CLE

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HRP-SAL

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HRP-SA

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EUR 269

HRP-HSA

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EUR 1114

HRP-HSA

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HRP-CAP

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HRP antibody

20C-CR7015GP 500 ul
EUR 80
Description: Goat polyclonal HRP antibody

HRP antibody

10R-1969 100 ul
EUR 381
Description: Mouse monoclonal HRP antibody

HRP antibody

10-3154 1 mg
EUR 208
Description: Mouse Monoclonal HRP antibody

HRP antibody

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EUR 392
Description: Mouse monoclonal HRP antibody

HRP antibody

20R-3010 100 µg
EUR 647
Description: Rabbit polyclonal HRP antibody

Digoxin-HRP

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EUR 398
Description: Conjugated Digoxin-HRP hapten

Methamphetamine-HRP

65-IM52 1 ml
EUR 444
Description: Conjugated Methamphetamine-HRP hapten

Phencyclidine-HRP

65-IP10 1 ml
EUR 440
Description: Conjugated Phencyclidine-HRP hapten

Progesterone-HRP

65-IP28 1 ml
EUR 480
Description: Conjugated Progesterone-HRP hapten

Triiodothyronine-HRP

65-IT35 50 units
EUR 381
Description: Conjugated Triiodothyronine-HRP hapten

Tobramycin-HRP

65-IT55 500 ug
EUR 500
Description: Conjugated Tobramycin-HRP Hapten

HRP Antibody

abx015753-100ul 100 ul
EUR 411
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Streptavidin (HRP)

abx670350-1mg 1 mg
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Streptavidin (HRP)

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EUR 467
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DHEA-HRP

80-1056 500 ul
EUR 812
Description: Conjugated DHEA-HRP hapten

Nortestosterone-HRP

80-1078 500 ul
EUR 137
Description: 17a-19-Nortestosterone 3 Conjugate for use in immunoassays

MDMA-HRP

80-1091 500 ul
EUR 136
Description: MDMA n Conjugate for use in immunoassays

CLAD-HRP

80-1092 500 ul
EUR 248
Description: CLAD 3 Conjugate for use in immunoassays

Nitrophenyl-HRP

80-1093 500 ul
EUR 417
Description: 4-Nitrophenyl Conjugate for use in immunoassays

Acepromazine-HRP

80-1097 500 ul
EUR 809
Description: Acepromazine Conjugate for use in immunoassays

Albendazole-HRP

80-1098 500 ul
EUR 809
Description: Albendazole Conjugate for use in immunoassays

Albendazole-HRP

80-1099 500 ul
EUR 248
Description: Albendazole Conjugate for use in immunoassays

Amikacin-HRP

80-1100 500 ul
EUR 1207
Description: Amikacin Conjugate for use in immunoassays

Ampicillin-HRP

80-1102 500 ul
EUR 137
Description: Ampicillin Conjugate for use in immunoassays

Amphetamine-HRP

80-1104 500 ul
EUR 248
Description: Amphetamine m Conjugate for use in immunoassays

Androstanedione-HRP

80-1105 500 ul
EUR 809
Description: Androstanedione 3 Conjugate for use in immunoassays

Apramycin-HRP

80-1106 500 ul
EUR 137
Description: Apramycin Conjugate for use in immunoassays

Avilamycin-HRP

80-1107 500 ul
EUR 607
Description: Avilamycin Conjugate for use in immunoassays

Azaperone-HRP

80-1108 500 ul
EUR 417
Description: Azaperone Conjugate for use in immunoassays

Bacitracin-HRP

80-1109 500 ul
EUR 417
Description: Bacitracin Conjugate for use in immunoassays

Boldenone-HRP

80-1110 500 ul
EUR 137
Description: Boldenone 17 Conjugate for use in immunoassays

Buprenorphine-HRP

80-1111 500 ul
EUR 137
Description: Buprenorphine 3 Conjugate for use in immunoassays

Norbuprenorphine-HRP

80-1112 500 ul
EUR 417
Description: Norbuprenorphine 3 Conjugate for use in immunoassays

Carazolol-HRP

80-1113 500 ul
EUR 402
Description: Carazolol Conjugate for use in immunoassays

Carbadox-HRP

80-1114 500 ul
EUR 137
Description: Carbadox Conjugate for use in immunoassays

Carbamazepine-HRP

80-1115 500 ul
EUR 248
Description: Carbamazepine Conjugate for use in immunoassays

Ceftiofur-HRP

80-1116 500 ul
EUR 137
Description: Ceftiofur Conjugate for use in immunoassays

Cephalexin-HRP

80-1117 500 ul
EUR 414
Description: Cephalexin Conjugate for use in immunoassays

Chloramphenicol-HRP

80-1118 500 ul
EUR 417
Description: Chloramphenicol Conjugate for use in immunoassays

Chlorpromazine-HRP

80-1120 500 ul
EUR 417
Description: Chlorpromazine Conjugate for use in immunoassays

Cimaterol-HRP

80-1121 500 ul
EUR 137
Description: Cimaterol Conjugate for use in immunoassays

Clenbuterol-HRP

80-1122 500 ul
EUR 403
Description: Clenbuterol N Conjugate for use in immunoassays

Closantel-HRP

80-1123 500 ul
EUR 248
Description: Closantel Conjugate for use in immunoassays

CLAD-HRP

80-1125 500 ul
EUR 248
Description: CLAD 3 Conjugate for use in immunoassays

Cocaine-HRP

80-1127 500 ul
EUR 137
Description: Cocaine Conjugate for use in immunoassays

Benzoylecgonine-HRP

80-1128 500 ul
EUR 137
Description: Benzoylecgonine COOH Conjugate for use in immunoassays

Cortisone-HRP

80-1131 500 ul
EUR 412
Description: Cortisone Conjugate for use in immunoassays

Creatinine-HRP

80-1133 500 ul
EUR 133
Description: Creatinine Conjugate for use in immunoassays

cAMP-HRP

80-1134 500 ul
EUR 248
Description: cAMP Conjugate for use in immunoassays

Cyclopentobarbital-HRP

80-1135 500 ul
EUR 248
Description: Cyclopentobarbital  5 Conjugate for use in immunoassays

Dapsone-HRP

80-1136 500 ul
EUR 137
Description: Dapsone Conjugate for use in immunoassays

Decoquinate-HRP

80-1137 500 ul
EUR 137
Description: Decoquinate Conjugate for use in immunoassays

Dehydromethyltestosterone-HRP

80-1139 500 ul
EUR 417
Description: Dehydromethyltestosterone 3 Conjugate for use in immunoassays

Desipramine-HRP

80-1140 500 ul
EUR 137
Description: Desipramine Conjugate for use in immunoassays

Desmethyllnoproxen-HRP

80-1141 500 ul
EUR 414
Description: Desmethyllnoproxen Conjugate for use in immunoassays

Dual-color tracing the whole body in vivo fluorescence imaging of xenografts done using live imaging system. transplanted tumor frozen section prepared for histological analysis, to detect the presence of tumor cells invade blood vessels and cell fusion. dual-color images were able to distinguish between tumor cells red and green host cells. Results of this research suggest that the dual-color fluorescence-tracing glioma orthotopic implantation model may be convenient to detect the location of tumors, angiogenesis, cell fusion, and the tumor microenvironment.