An Analysis of the Practicalities of Multi-Color Nanoparticle Cellular Bar-Coding.

Many applications in biomedical research requires long-range identification and tracking of cells over time. In previous work we have shown that with sequential doses of a population of cells with nanoparticles of different emission wavelengths is possible to use a random number of nanoparticles loaded vesicles produced by cells as a barcode for individual cells in the population.

In this paper we develop a simple model to describe the amount of code that can be generated by using this sequential loading protocol. This methodology is validated in comparison with experiment and then used to predict the effects of various number of colors used to encode the cell and also to assess the effects of misread codes due to errors in the imaging cellular vesicles.

A Quick, Easy and Customizable Eight-color flow cytometric Method for Mobile Content Analysis of Bronchoalveolar Lavage Fluids in Mouse.

An Analysis of the Practicalities of Multi-Color Nanoparticle Cellular Bar-Coding.
An Analysis of the Practicalities of Multi-Color Nanoparticle Cellular Bar-Coding.

Cell composition of bronchoalveolar lavage fluid (BAL) is an important indicator of airway inflammation. It is generally determined by cytocentrifuging leukocytes on a slide, then staining, identify, and count them as eosinophils, neutrophils, macrophages, or lymphocytes according to morphological criteria under a light microscope, where it is not always easy to distinguish macrophages from the lymphocytes.

We describe here a single-step, easy to use, and easy-to-customize 8-color flow cytometric method to perform differential cell count and compare it with the number of morphology on stained cytospins. This method identifies BAL cells by one step immunolabeling simultaneous procedure using antibodies to identify T cells, B cells, neutrophils, eosinophils, and macrophages.

morphological analysis of flow-sorted cell subsets are used to validate this protocol. An important advantage of flow cytometry basic protocol is the ability to customize by adding antibodies to study the expression of receptors on the cell surface of leukocytes and identify subclasses of inflammatory cells as needed. © 2017 by John Wiley & Sons, Inc.

The advantage of dual-color fluorescence-tracing glioma orthotopic implantation model: Detecting the location of the tumor, angiogenesis, cell fusion and the tumor microenvironment.

Various organs of the body have different microenvironments with diverse biological characteristics that can influence tumor growth in themselves. However, the mechanisms underlying the interaction between tumor cells and the host is not currently well understood.

In this study, glioma model is the implantation of orthotopic fluorescence-tracing dual-color developed, in which the C6 cells rat glioma is labeled with a fluorescent dye red CM-Dil and glioma cells SU3 man stably express the protein fluorescence red, were inoculated into the right caudate nucleus of GMOs female C57BL / 6 nude mice revealed enhanced green fluorescent protein.

Prediluted for Immunohistochemistry

RP-4003-04 ImmunoBioscience 7.0 ml 79.65 EUR

Tn, Prediluted for Immunohistochemistry

MM-1018-04 ImmunoBioscience 7.0 ml 120.05 EUR

Tg, Prediluted for Immunohistochemistry

MM-1021-04 ImmunoBioscience 7.0 ml 120.05 EUR

Tg, Prediluted for Immunohistochemistry

RP-4009-04 ImmunoBioscience 7.0 ml 79.65 EUR

K Prediluted for Immunohistochemistry

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EMA, Prediluted for Immunohistochemistry

MM-1001-04 ImmunoBioscience 7.0 ml 120.05 EUR

hCG, Prediluted for Immunohistochemistry

MM-1005-04 ImmunoBioscience 7.0 ml 120.05 EUR

ESA, Prediluted for Immunohistochemistry

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p53, Prediluted for Immunohistochemistry

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STn, Prediluted for Immunohistochemistry

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TSH, Prediluted for Immunohistochemistry

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CTP, Prediluted for Immunohistochemistry

RP-4004-04 ImmunoBioscience 7.0 ml 79.65 EUR

CEA, Prediluted for Immunohistochemistry

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PSA, Prediluted for Immunohistochemistry

RP-4010-04 ImmunoBioscience 7.0 ml 79.65 EUR

MBP, Prediluted for Immunohistochemistry

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AFP, Prediluted for Immunohistochemistry

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HiPer® Immunohistochemistry Teaching Kit

HTI023-5PR EWC Diagnostics 1 unit 144.14 EUR
Description: HiPer® Immunohistochemistry Teaching Kit

AαSM, Prediluted for Immunohistochemistry

MM-1004-04 ImmunoBioscience 7.0 ml 120.05 EUR

Brdu, Prediluted for Immunohistochemistry

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PCNA, Prediluted for Immunohistochemistry

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MUC2, Prediluted for Immunohistochemistry

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S100, Prediluted for Immunohistochemistry

RP-4014-04 ImmunoBioscience 7.0 ml 79.65 EUR

GFAP, Prediluted for Immunohistochemistry

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A1AT, Prediluted for Immunohistochemistry

RP-4019-04 ImmunoBioscience 7.0 ml 79.65 EUR

CK 19, Prediluted for Immunohistochemistry

MM-1002-04 ImmunoBioscience 7.0 ml 120.05 EUR

CK 18, Prediluted for Immunohistochemistry

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HBcAg, Prediluted for Immunohistochemistry

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CK AE1, Prediluted for Immunohistochemistry

MM-1010-04 ImmunoBioscience 7.0 ml 120.05 EUR

CK AE3, Prediluted for Immunohistochemistry

MM-1011-04 ImmunoBioscience 7.0 ml 120.05 EUR

S 100, Prediluted for Immunohistochemistry

MM-1017-04 ImmunoBioscience 7.0 ml 120.05 EUR

Lambda, Prediluted for Immunohistochemistry

RP-4021-04 ImmunoBioscience 7.0 ml 79.65 EUR

Desmin, Prediluted for Immunohistochemistry

RP-4023-04 ImmunoBioscience 7.0 ml 79.65 EUR

CK 8+18, Prediluted for Immunohistochemistry

MM-1007-04 ImmunoBioscience 7.0 ml 120.05 EUR

CK 10+13, Prediluted for Immunohistochemistry

MM-1008-04 ImmunoBioscience 7.0 ml 120.05 EUR

Insulin Prediluted for Immunohistochemistry

RP-4012-04 ImmunoBioscience 7.0 ml 79.65 EUR

PR Immunohistochemistry control tissue array

PM2-PR TissueArray each 78 EUR
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Ferritin, Prediluted for Immunohistochemistry

RP-4001-04 ImmunoBioscience 7.0 ml 79.65 EUR

Vimentin, Prediluted for Immunohistochemistry

RP-4002-04 ImmunoBioscience 7.0 ml 79.65 EUR

Lysozyme, Prediluted for Immunohistochemistry

RP-4018-04 ImmunoBioscience 7.0 ml 79.65 EUR

Calcitonin, Prediluted for Immunohistochemistry

RP-4007-04 ImmunoBioscience 7.0 ml 79.65 EUR

Ubiquintin, Prediluted for Immunohistochemistry

RP-4011-04 ImmunoBioscience 7.0 ml 79.65 EUR

CK cocktail. Prediluted for Immunohistochemistry

MM-1012-04 ImmunoBioscience 7.0 ml 191.1 EUR

Transferrin, Prediluted for Immunohistochemistry

RP-4022-04 ImmunoBioscience 7.0 ml 79.65 EUR

Sialosyl Le (a), Prediluted for Immunohistochemistry

MM-1003-04 ImmunoBioscience 7.0 ml 120.05 EUR

Synaptophysin, Prediluted for Immunohistochemistry

RP-4008-04 ImmunoBioscience 7.0 ml 79.65 EUR

Normal Rabbit Control Serum (for immunohistochemistry)

NRS-IHC PHOENIX PEPTIDE 500 μl 267.84 EUR

GWB-55335F-3ML - DAB Immunohistochemistry Substrate

GWB-55335F-3ML Aviva Systems Biology 3ml 143 EUR

Myeloperoxidase (MPO), Prediluted for Immunohistochemistry

RP-4025-04 ImmunoBioscience 7.0 ml 79.65 EUR

Luteinizing Hormone (LH), Prediluted for Immunohistochemistry

RP-4024-04 ImmunoBioscience 7.0 ml 79.65 EUR

Cytokeratin AE1 and AE3, Prediluted for Immunohistochemistry

MM-1022-04 ImmunoBioscience 7.0 ml 136.15 EUR

Breast cancer with ER Immunohistochemistry control tissue array

PM2a-ER TissueArray each 78 EUR
Description: Breast cancer with ER Immunohistochemistry control tissue array, including pathology grade, TNM, clinical stage and IHC results of ER/PR/Her-2/Ki67, 10 cases/10 cores, replacing PM2-ER

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OOMB00003-10ML Aviva Systems Biology 10mL 79 EUR

OOMB00006-100ML - 10X Antigen Retrieval Solution pH 6.0 for Immunohistochemistry

OOMB00006-100ML Aviva Systems Biology 100mL 79 EUR

OOMB00007-100ML - 10X Antigen Retrieval Solution pH 9.0 for Immunohistochemistry

OOMB00007-100ML Aviva Systems Biology 100mL 79 EUR

OOMB00004-10ML - Permeabilization Buffer for Immunocytochemistry and Immunohistochemistry

OOMB00004-10ML Aviva Systems Biology 10mL 79 EUR

Epidermal Growth Factor Receptor, phosphorylated (Tyr1173) (erbB, EGFR), Immunohistochemistry Controls

MBS652639-5Slides MyBiosource 5Slides 460 EUR

Epidermal Growth Factor Receptor, phosphorylated (Tyr1173) (erbB, EGFR), Immunohistochemistry Controls

MBS652639-5x5Slides MyBiosource 5x5Slides 1920 EUR

MAP Kinase p44/42, phosphorylated (Thr202, Tyr204), Immunohistochemistry Controls (MAPK3/MAPK1, Mitogen-activated Protein Kinase 1, MAP Kinase 1, MAPK 1, ERT1, Extracellular Signal-regulated Kinase 2, ERK-2, MAP Kinase Isoform p42, p42-MAPK, Mitogen-activ

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MAP Kinase p44/42, phosphorylated (Thr202, Tyr204), Immunohistochemistry Controls (MAPK3/MAPK1, Mitogen-activated Protein Kinase 1, MAP Kinase 1, MAPK 1, ERT1, Extracellular Signal-regulated Kinase 2, ERK-2, MAP Kinase Isoform p42, p42-MAPK, Mitogen-activ

MBS652636-5x5Slides MyBiosource 5x5Slides 1900 EUR

Super Plus High Sensitive and Rapid Immunohistochemical Kit (pH9.0)

MBS2567656-10mL MyBiosource 10mL 310 EUR

Super Plus High Sensitive and Rapid Immunohistochemical Kit (pH9.0)

MBS2567656-3mL MyBiosource 3mL 185 EUR

Super Plus High Sensitive and Rapid Immunohistochemical Kit (pH9.0)

MBS2567656-5x10mL MyBiosource 5x10mL 1370 EUR

Super Plus High Sensitive and Rapid Immunohistochemical Kit (pH6.0)

MBS2567657-10mL MyBiosource 10mL 310 EUR

Super Plus High Sensitive and Rapid Immunohistochemical Kit (pH6.0)

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Super Plus High Sensitive and Rapid Immunohistochemical Kit (pH6.0)

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Super PlusTMHigh Sensitive and Rapid Immunohistochemical Kit (pH9.0)

E-IR-R220-10mL Elabscience Biotech 10mL 325 EUR

Super PlusTMHigh Sensitive and Rapid Immunohistochemical Kit (pH9.0)

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Super PlusTMHigh Sensitive and Rapid Immunohistochemical Kit (pH9.0)

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Super PlusTMHigh Sensitive and Rapid Immunohistochemical Kit (pH6.0)

E-IR-R221-10mL Elabscience Biotech 10mL 325 EUR

Super PlusTMHigh Sensitive and Rapid Immunohistochemical Kit (pH6.0)

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ImmunoHistoMount™

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ImmunoHistoMount™

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IgE (Immunoglobulin E) (HRP)

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IgE (Immunoglobulin E) (HRP)

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Immunoglobulin G (IgG) Fc -HRP

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Immunoglobulin G (IgG), Rat -HRP

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Immunoglobulin E (IgE), Dog -HRP

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Immunoglobulin M (IgM), Pig -HRP

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Immunoglobulin G (IgG), Goat -HRP

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Immunoglobulin G (IgG), Goat -HRP

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Immunoglobulin G (IgG), Human -HRP

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Immunoglobulin G (IgG), Mouse -HRP

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Immunoglobulin G (IgG), Mouse -HRP

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Yolk Immunoglobulin Antibody (HRP)

abx108951-500ug Abbexa 500 ug 360 EUR

Yolk Immunoglobulin Antibody (HRP)

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Yolk Immunoglobulin Antibody (HRP)

abx108951-20g Abbexa 20 µg 250 EUR

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Immunoglobulin G (IgG), Rabbit -HRP

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Immunoglobulin M (IgM), Bovine -HRP

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Immunoglobulin G3 (IgG3), Mouse -HRP

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Immunoglobulin G1 (IgG1), Mouse -HRP

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Immunoglobulin G2 (IgG2), Bovine -HRP

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Immunoglobulin G1 (IgG1), Bovine -HRP

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Immunoglobulin M (IgM) Antibody (HRP)

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20-abx271010 Abbexa
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Immunoglobulin G (IgG) Antibody (HRP)

abx271010-1ml Abbexa 1 ml 775 EUR

Immunoglobulin G (IgG) Antibody (HRP)

abx271010-200l Abbexa 200 µl 350 EUR

Immunoglobulin M (IgM) Antibody (HRP)

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Immunoglobulin M (IgM) Antibody (HRP)

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Immunoglobulin G (IgG) (H+L), Cat -HRP

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Immunoglobulin G (IgG) (H&L) , Dog -HRP

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Immunoglobulin G (IgG) & IgM, Mouse -HRP

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Immunoglobulin G (IgG) (H+L), Goat -HRP

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Immunoglobulin G (IgG) H+L, Bird -HRP

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Immunoglobulin G (IgG) (H+L), Horse -HRP

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Immunoglobulin G (IgG) (H&L), Sheep -HRP

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Immunoglobulin G (IgG) (H&L), Human -HRP

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Immunoglobulin G (IgG) (H+L), Swine -HRP

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Immunoglobulin M (IgM) Fc5u, Fab`2 -HRP

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Immunoglobulin G (IgG) (H&L), Mouse -HRP

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Immunoglobulin G2a (IgG2a) Antibody (HRP)

20-abx319024 Abbexa
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Immunoglobulin G2a (IgG2a) Antibody (HRP)

abx319024-100l Abbexa 100 µl 250 EUR

Immunoglobulin G2a (IgG2a) Antibody (HRP)

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Immunoglobulin G2 Alpha (IgG2a), Rat -HRP

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Immunoglobulin IgG, IgA, IgM, Monkey -HRP

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Immunoglobulin G (IgG) (H+L), Bovine -HRP

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Immunoglobulin G (IgG) Fab\ 2, Mouse -HRP

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Immunoglobulin G (IgG) (H+L), Bovine -HRP

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Immunogenic protein MPT64 Antibody (HRP)

20-abx109171 Abbexa
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Immunogenic protein MPT63 Antibody (HRP)

20-abx109229 Abbexa
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Immunogenic protein MPT64 Antibody (HRP)

20-abx109243 Abbexa
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Immunogenic protein MPT64 Antibody (HRP)

abx109171-100l Abbexa 100 µl 162.5 EUR

Immunogenic protein MPT63 Antibody (HRP)

abx109229-100l Abbexa 100 µl 162.5 EUR

Immunogenic protein MPT64 Antibody (HRP)

abx109243-100l Abbexa 100 µl 162.5 EUR

Immunoglobulin Y (IgY) (H&L), Chicken -HRP

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Immunoglobulin G (IgG) Fab`2, Bovine -HRP

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Immunoglobulin G (IgG) H&L, Chicken -HRP

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Immunoglobulin G (IgG) Fab\ 2, Rabbit -HRP

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Immunoglobulin G2 Alpha (IgG2a), Mouse -HRP

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Immunoglobulin E (IgE) Human -HRP, Antibody

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Immunoglobulin M Mu Chain (IgM), Monkey -HRP

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Feline Immunodeficiency Virus, p24 (FIV) (HRP)

MBS6250869-01mL MyBiosource 0.1(mL 1025 EUR

Feline Immunodeficiency Virus, p24 (FIV) (HRP)

MBS6250869-5x01mL MyBiosource 5x0.1mL 4470 EUR

Immunoglobulin G (IgG) H&L, Guinea Pig -HRP

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Immunoglobulin IgG, IgA ,IgM H&L, Mouse -HRP

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Immunoglobulin IgA & IgG & IgM (H+L), Human -HRP

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Immunoglobulin G (IgG) (H&L), Guinea Pig -HRP

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PIGR (Polymeric Immunoglobulin Receptor) (HRP).

MBS6448399-01mL MyBiosource 0.1mL 950 EUR

PIGR (Polymeric Immunoglobulin Receptor) (HRP).

MBS6448399-5x01mL MyBiosource 5x0.1mL 4120 EUR

Polymeric Immunoglobulin Receptor (PIGR) (HRP)

MBS6403399-01mL MyBiosource 0.1mL 800 EUR

Polymeric Immunoglobulin Receptor (PIGR) (HRP)

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Immunoglobulin G (IgG), Armenian Hamster -HRP

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Feline Immunodeficiency Virus, gp95 (FIV) (HRP)

MBS6250868-01mL MyBiosource 0.1(mL 1005 EUR

Feline Immunodeficiency Virus, gp95 (FIV) (HRP)

MBS6250868-5x01mL MyBiosource 5x0.1mL 4365 EUR

Immunoglobulin Y (IgY) (IgG) (H+L), Chicken -HRP

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HIV-1 (Human Immunodeficiency Virus-1) (HRP)

MBS6125628-01mL MyBiosource 0.1(mL 765 EUR

HIV-1 (Human Immunodeficiency Virus-1) (HRP)

MBS6125628-5x01mL MyBiosource 5x0.1mL 3300 EUR

Immunogenic Protein MPT64 (mpt64) Antibody (HRP)

abx345693-100g Abbexa 100 µg 362.5 EUR

Immunogenic Protein MPT64 (mpt64) Antibody (HRP)

abx345693-20g Abbexa 20 µg 162.5 EUR

Immunogenic Protein MPT64 (mpt64) Antibody (HRP)

abx345693-50g Abbexa 50 µg 250 EUR

Feline Immunodeficiency Virus, p24 gag (FIV) (HRP)

MBS6250870-01mL MyBiosource 0.1(mL 1140 EUR

Feline Immunodeficiency Virus, p24 gag (FIV) (HRP)

MBS6250870-5x01mL MyBiosource 5x0.1mL 4970 EUR

Immunoglobulin G (IgG) (H+L), Syrian Hamster -HRP

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Polymeric immunoglobulin receptor Antibody (HRP)

20-abx109077 Abbexa
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Polymeric immunoglobulin receptor Antibody (HRP)

abx109077-100l Abbexa 100 µl 162.5 EUR

Dual-color tracing the whole body in vivo fluorescence imaging of xenografts done using live imaging system. transplanted tumor frozen section prepared for histological analysis, to detect the presence of tumor cells invade blood vessels and cell fusion. dual-color images were able to distinguish between tumor cells red and green host cells. Results of this research suggest that the dual-color fluorescence-tracing glioma orthotopic implantation model may be convenient to detect the location of tumors, angiogenesis, cell fusion, and the tumor microenvironment.