An Analysis of the Practicalities of Multi-Color Nanoparticle Cellular Bar-Coding.

Many applications in biomedical research requires long-range identification and tracking of cells over time. In previous work we have shown that with sequential doses of a population of cells with nanoparticles of different emission wavelengths is possible to use a random number of nanoparticles loaded vesicles produced by cells as a barcode for individual cells in the population.

In this paper we develop a simple model to describe the amount of code that can be generated by using this sequential loading protocol. This methodology is validated in comparison with experiment and then used to predict the effects of various number of colors used to encode the cell and also to assess the effects of misread codes due to errors in the imaging cellular vesicles.

A Quick, Easy and Customizable Eight-color flow cytometric Method for Mobile Content Analysis of Bronchoalveolar Lavage Fluids in Mouse.

An Analysis of the Practicalities of Multi-Color Nanoparticle Cellular Bar-Coding.
An Analysis of the Practicalities of Multi-Color Nanoparticle Cellular Bar-Coding.

Cell composition of bronchoalveolar lavage fluid (BAL) is an important indicator of airway inflammation. It is generally determined by cytocentrifuging leukocytes on a slide, then staining, identify, and count them as eosinophils, neutrophils, macrophages, or lymphocytes according to morphological criteria under a light microscope, where it is not always easy to distinguish macrophages from the lymphocytes.

We describe here a single-step, easy to use, and easy-to-customize 8-color flow cytometric method to perform differential cell count and compare it with the number of morphology on stained cytospins. This method identifies BAL cells by one step immunolabeling simultaneous procedure using antibodies to identify T cells, B cells, neutrophils, eosinophils, and macrophages.

morphological analysis of flow-sorted cell subsets are used to validate this protocol. An important advantage of flow cytometry basic protocol is the ability to customize by adding antibodies to study the expression of receptors on the cell surface of leukocytes and identify subclasses of inflammatory cells as needed. © 2017 by John Wiley & Sons, Inc.

The advantage of dual-color fluorescence-tracing glioma orthotopic implantation model: Detecting the location of the tumor, angiogenesis, cell fusion and the tumor microenvironment.

Various organs of the body have different microenvironments with diverse biological characteristics that can influence tumor growth in themselves. However, the mechanisms underlying the interaction between tumor cells and the host is not currently well understood.

In this study, glioma model is the implantation of orthotopic fluorescence-tracing dual-color developed, in which the C6 cells rat glioma is labeled with a fluorescent dye red CM-Dil and glioma cells SU3 man stably express the protein fluorescence red, were inoculated into the right caudate nucleus of GMOs female C57BL / 6 nude mice revealed enhanced green fluorescent protein.

Tg, Prediluted for Immunohistochemistry

MM-1021-04 ImmunoBioscience 7.0 ml 120.05 EUR

Tg, Prediluted for Immunohistochemistry

RP-4009-04 ImmunoBioscience 7.0 ml 79.65 EUR

K Prediluted for Immunohistochemistry

RP-4020-04 ImmunoBioscience 7.0 ml 79.65 EUR

EMA, Prediluted for Immunohistochemistry

MM-1001-04 ImmunoBioscience 7.0 ml 120.05 EUR

hCG, Prediluted for Immunohistochemistry

MM-1005-04 ImmunoBioscience 7.0 ml 120.05 EUR

ESA, Prediluted for Immunohistochemistry

MM-1014-04 ImmunoBioscience 7.0 ml 120.05 EUR

p53, Prediluted for Immunohistochemistry

MM-1015-04 ImmunoBioscience 7.0 ml 120.05 EUR

STn, Prediluted for Immunohistochemistry

MM-1019-04 ImmunoBioscience 7.0 ml 120.05 EUR

TSH, Prediluted for Immunohistochemistry

MM-1020-04 ImmunoBioscience 7.0 ml 120.05 EUR

CTP, Prediluted for Immunohistochemistry

RP-4004-04 ImmunoBioscience 7.0 ml 79.65 EUR

CEA, Prediluted for Immunohistochemistry

RP-4005-04 ImmunoBioscience 7.0 ml 79.65 EUR

PSA, Prediluted for Immunohistochemistry

RP-4010-04 ImmunoBioscience 7.0 ml 79.65 EUR

MBP, Prediluted for Immunohistochemistry

RP-4015-04 ImmunoBioscience 7.0 ml 79.65 EUR

AFP, Prediluted for Immunohistochemistry

RP-4017-04 ImmunoBioscience 7.0 ml 79.65 EUR

HiPer® Immunohistochemistry Teaching Kit

HTI023-5PR EWC Diagnostics 1 unit 144.14 EUR
Description: HiPer® Immunohistochemistry Teaching Kit

AαSM, Prediluted for Immunohistochemistry

MM-1004-04 ImmunoBioscience 7.0 ml 120.05 EUR

Brdu, Prediluted for Immunohistochemistry

MM-1006-04 ImmunoBioscience 7.0 ml 120.05 EUR

PCNA, Prediluted for Immunohistochemistry

MM-1016-04 ImmunoBioscience 7.0 ml 120.05 EUR

MUC2, Prediluted for Immunohistochemistry

RP-4013-04 ImmunoBioscience 7.0 ml 79.65 EUR

S100, Prediluted for Immunohistochemistry

RP-4014-04 ImmunoBioscience 7.0 ml 79.65 EUR

GFAP, Prediluted for Immunohistochemistry

RP-4016-04 ImmunoBioscience 7.0 ml 79.65 EUR

A1AT, Prediluted for Immunohistochemistry

RP-4019-04 ImmunoBioscience 7.0 ml 79.65 EUR

CK 19, Prediluted for Immunohistochemistry

MM-1002-04 ImmunoBioscience 7.0 ml 120.05 EUR

CK 18, Prediluted for Immunohistochemistry

MM-1009-04 ImmunoBioscience 7.0 ml 120.05 EUR

HBcAg, Prediluted for Immunohistochemistry

RP-4006-04 ImmunoBioscience 7.0 ml 79.65 EUR

CK AE1, Prediluted for Immunohistochemistry

MM-1010-04 ImmunoBioscience 7.0 ml 120.05 EUR

CK AE3, Prediluted for Immunohistochemistry

MM-1011-04 ImmunoBioscience 7.0 ml 120.05 EUR

S 100, Prediluted for Immunohistochemistry

MM-1017-04 ImmunoBioscience 7.0 ml 120.05 EUR

Lambda, Prediluted for Immunohistochemistry

RP-4021-04 ImmunoBioscience 7.0 ml 79.65 EUR

Desmin, Prediluted for Immunohistochemistry

RP-4023-04 ImmunoBioscience 7.0 ml 79.65 EUR

CK 8+18, Prediluted for Immunohistochemistry

MM-1007-04 ImmunoBioscience 7.0 ml 120.05 EUR

CK 10+13, Prediluted for Immunohistochemistry

MM-1008-04 ImmunoBioscience 7.0 ml 120.05 EUR

Insulin Prediluted for Immunohistochemistry

RP-4012-04 ImmunoBioscience 7.0 ml 79.65 EUR

PR Immunohistochemistry control tissue array

PM2-PR TissueArray each 78 EUR
Description: PR Immunohistochemistry control tissue array, including TNM and pathology grade of breast cancer, 11 cases/11 cores

Ferritin, Prediluted for Immunohistochemistry

RP-4001-04 ImmunoBioscience 7.0 ml 79.65 EUR

Vimentin, Prediluted for Immunohistochemistry

RP-4002-04 ImmunoBioscience 7.0 ml 79.65 EUR

Lysozyme, Prediluted for Immunohistochemistry

RP-4018-04 ImmunoBioscience 7.0 ml 79.65 EUR

Calcitonin, Prediluted for Immunohistochemistry

RP-4007-04 ImmunoBioscience 7.0 ml 79.65 EUR

Ubiquintin, Prediluted for Immunohistochemistry

RP-4011-04 ImmunoBioscience 7.0 ml 79.65 EUR

CK cocktail. Prediluted for Immunohistochemistry

MM-1012-04 ImmunoBioscience 7.0 ml 191.1 EUR

Transferrin, Prediluted for Immunohistochemistry

RP-4022-04 ImmunoBioscience 7.0 ml 79.65 EUR

Sialosyl Le (a), Prediluted for Immunohistochemistry

MM-1003-04 ImmunoBioscience 7.0 ml 120.05 EUR

Synaptophysin, Prediluted for Immunohistochemistry

RP-4008-04 ImmunoBioscience 7.0 ml 79.65 EUR

Normal Rabbit Control Serum (for immunohistochemistry)

NRS-IHC PHOENIX PEPTIDE 500 μl 267.84 EUR

GWB-55335F-3ML - DAB Immunohistochemistry Substrate

GWB-55335F-3ML Aviva Systems Biology 3ml 143 EUR

Myeloperoxidase (MPO), Prediluted for Immunohistochemistry

RP-4025-04 ImmunoBioscience 7.0 ml 79.65 EUR

Luteinizing Hormone (LH), Prediluted for Immunohistochemistry

RP-4024-04 ImmunoBioscience 7.0 ml 79.65 EUR

Cytokeratin AE1 and AE3, Prediluted for Immunohistochemistry

MM-1022-04 ImmunoBioscience 7.0 ml 136.15 EUR

Breast cancer with ER Immunohistochemistry control tissue array

PM2a-ER TissueArray each 78 EUR
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OOMB00003-10ML - Blocking Buffer for Immunocytochemistry and Immunohistochemistry

OOMB00003-10ML Aviva Systems Biology 10mL 79 EUR

OOMB00006-100ML - 10X Antigen Retrieval Solution pH 6.0 for Immunohistochemistry

OOMB00006-100ML Aviva Systems Biology 100mL 79 EUR

OOMB00007-100ML - 10X Antigen Retrieval Solution pH 9.0 for Immunohistochemistry

OOMB00007-100ML Aviva Systems Biology 100mL 79 EUR

OOMB00004-10ML - Permeabilization Buffer for Immunocytochemistry and Immunohistochemistry

OOMB00004-10ML Aviva Systems Biology 10mL 79 EUR

Super PlusTMHigh Sensitive and Rapid Immunohistochemical Kit (pH9.0)

E-IR-R220-10mL Elabscience Biotech 10mL 325 EUR

Super PlusTMHigh Sensitive and Rapid Immunohistochemical Kit (pH9.0)

E-IR-R220-3mL Elabscience Biotech 3mL 125 EUR

Super PlusTMHigh Sensitive and Rapid Immunohistochemical Kit (pH9.0)

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Super PlusTMHigh Sensitive and Rapid Immunohistochemical Kit (pH6.0)

E-IR-R221-10mL Elabscience Biotech 10mL 325 EUR

Super PlusTMHigh Sensitive and Rapid Immunohistochemical Kit (pH6.0)

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Super PlusTMHigh Sensitive and Rapid Immunohistochemical Kit (pH6.0)

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ImmunoHistozyme

AR-6547-01 ImmunoBioscience 15 ml 47.75 EUR

ImmunoHistozyme

AR-6547-02 ImmunoBioscience 100 ml 95.55 EUR

ImmunoHistoMount™

AR-6503-01 ImmunoBioscience 30 ml 60.05 EUR

ImmunoHistoMount™

AR-6503-02 ImmunoBioscience 100 ml 111.65 EUR

ImmunoHistoMount™

AR-6503-03 ImmunoBioscience 250 ml 203.35 EUR

ImmunoHistoMount™

AR-6503-05 ImmunoBioscience 1000 ml 556.5 EUR

Immunoglobulin G (IgG) Fc -HRP

GWB-367ACF GenWay Biotech 2 mg Ask for price

Immunoglobulin G (IgG), Rat -HRP

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Immunoglobulin E (IgE), Dog -HRP

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Immunoglobulin A (IgA), Pig -HRP

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Immunoglobulin M (IgM), Pig -HRP

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Immunoglobulin G (IgG), Goat -HRP

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Immunoglobulin G (IgG), Goat -HRP

GWB-F617F6 GenWay Biotech 2 mg Ask for price

Immunoglobulin G (IgG), Mouse -HRP

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Immunoglobulin G (IgG), Human -HRP

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Immunoglobulin G (IgG), Mouse -HRP

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Immunoglobulin G (IgG)-Fc, Pig -HRP

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Yolk Immunoglobulin Antibody (HRP)

abx108951-500ug Abbexa 500 ug 360 EUR

Yolk Immunoglobulin Antibody (HRP)

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abx108951-20g Abbexa 20 µg 250 EUR

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Immunoglobulin G1 (IgG1), Bovine -HRP

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Immunoglobulin G (IgG) Antibody (HRP)

20-abx271010 Abbexa
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Immunoglobulin M (IgM) Antibody (HRP)

abx139562-01mg Abbexa 0.1 mg 526.8 EUR

Immunoglobulin G (IgG) Antibody (HRP)

abx271010-1ml Abbexa 1 ml 775 EUR

Immunoglobulin G (IgG) Antibody (HRP)

abx271010-200l Abbexa 200 µl 350 EUR

Immunoglobulin M (IgM) Antibody (HRP)

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Immunoglobulin M (IgM) Antibody (HRP)

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Immunoglobulin G (IgG) (H+L), Cat -HRP

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Immunoglobulin G (IgG) (H&L) , Dog -HRP

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Immunoglobulin G (IgG) & IgM, Mouse -HRP

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Immunoglobulin G (IgG) (H+L), Goat -HRP

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Immunoglobulin G (IgG) H+L, Bird -HRP

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Immunoglobulin G (IgG) (H&L), Human -HRP

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Immunoglobulin G (IgG) (H&L), Sheep -HRP

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Immunoglobulin G (IgG) (H+L), Horse -HRP

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Immunoglobulin G (IgG) (H+L), Swine -HRP

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Immunoglobulin M (IgM) Fc5u, Fab`2 -HRP

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Immunoglobulin G (IgG) (H&L), Mouse -HRP

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Immunoglobulin G2 Alpha (IgG2a), Rat -HRP

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Immunoglobulin IgG, IgA, IgM, Monkey -HRP

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Immunoglobulin G2a (IgG2a) Antibody (HRP)

20-abx319024 Abbexa
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Immunoglobulin G2a (IgG2a) Antibody (HRP)

abx319024-100l Abbexa 100 µl 250 EUR

Immunoglobulin G2a (IgG2a) Antibody (HRP)

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Immunoglobulin G (IgG) (H+L), Bovine -HRP

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Immunoglobulin G (IgG) Fab\ 2, Mouse -HRP

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Immunoglobulin G (IgG) (H+L), Rabbit -HRP

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Immunoglobulin G (IgG) (H+L), Rabbit -HRP

GWB-703BE8 GenWay Biotech 2 ml Ask for price

Immunoglobulin G (IgG) (H+L), Bovine -HRP

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Immunogenic protein MPT64 Antibody (HRP)

20-abx109171 Abbexa
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Immunogenic protein MPT63 Antibody (HRP)

20-abx109229 Abbexa
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Immunogenic protein MPT64 Antibody (HRP)

20-abx109243 Abbexa
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Immunogenic protein MPT64 Antibody (HRP)

abx109171-100l Abbexa 100 µl 162.5 EUR

Immunogenic protein MPT63 Antibody (HRP)

abx109229-100l Abbexa 100 µl 162.5 EUR

Immunogenic protein MPT64 Antibody (HRP)

abx109243-100l Abbexa 100 µl 162.5 EUR

Immunoglobulin G (IgG) Fab`2, Bovine -HRP

GWB-1EBC75 GenWay Biotech 2 mg Ask for price

Immunoglobulin G (IgG) H&L, Chicken -HRP

GWB-2B971A GenWay Biotech 1.5 mg Ask for price

Immunoglobulin Y (IgY) (H&L), Chicken -HRP

GWB-59DD7C GenWay Biotech 0.5 ml Ask for price

Immunoglobulin G (IgG) Fab\ 2, Rabbit -HRP

GWB-A2FC28 GenWay Biotech 0.3 ml Ask for price

Immunoglobulin G2 Alpha (IgG2a), Mouse -HRP

GWB-42762B GenWay Biotech 1 ml Ask for price

Immunoglobulin E (IgE) Human -HRP, Antibody

GWB-BC8EC1 GenWay Biotech 1 mg Ask for price

Immunoglobulin M Mu Chain (IgM), Monkey -HRP

GWB-22C2FA GenWay Biotech 1 mg Ask for price

Immunoglobulin G (IgG) H&L, Guinea Pig -HRP

GWB-5018A5 GenWay Biotech 2 mg Ask for price

Immunoglobulin IgG, IgA ,IgM H&L, Mouse -HRP

GWB-169006 GenWay Biotech 2 mg Ask for price

Immunoglobulin IgA & IgG & IgM (H+L), Human -HRP

GWB-A7CA8D GenWay Biotech 2 ml Ask for price

Immunoglobulin G (IgG) (H&L), Guinea Pig -HRP

GWB-E77E10 GenWay Biotech 0.5 ml Ask for price

Immunoglobulin Y (IgY) (IgG) (H+L), Chicken -HRP

GWB-8B76BA GenWay Biotech 1.5 ml Ask for price

Immunoglobulin G (IgG), Armenian Hamster -HRP

GWB-2CDF79 GenWay Biotech 0.5 ml Ask for price

Immunoglobulin G (IgG), Armenian Hamster -HRP

GWB-70CD24 GenWay Biotech 1.5 ml Ask for price

Immunoglobulin G (IgG) (Heavy Chain), Bovine -HRP

GWB-1C72DC GenWay Biotech 1 mg Ask for price

Immunogenic Protein MPT64 (mpt64) Antibody (HRP)

abx345693-100g Abbexa 100 µg 362.5 EUR

Immunogenic Protein MPT64 (mpt64) Antibody (HRP)

abx345693-20g Abbexa 20 µg 162.5 EUR

Immunogenic Protein MPT64 (mpt64) Antibody (HRP)

abx345693-50g Abbexa 50 µg 250 EUR

Immunoglobulin G (IgG) (H+L), Syrian Hamster -HRP

GWB-8EF017 GenWay Biotech 1 ml Ask for price

Immunoglobulin IgM u Chain Specific, Mouse -HRP

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Polymeric immunoglobulin receptor Antibody (HRP)

20-abx109077 Abbexa
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Polymeric immunoglobulin receptor Antibody (HRP)

abx109077-100l Abbexa 100 µl 162.5 EUR

Immunoglobulin kappa constant (IGKC) Antibody (HRP)

abx343594-100l Abbexa 100 µl 250 EUR

Immunoglobulin kappa constant (IGKC) Antibody (HRP)

abx343594-50l Abbexa 50 µl 162.5 EUR

Immuno HRP-AEC,ready to use IHC kit,anti-chicken IgY (H+L)

IH-8043-15 ImmunoBioscience each reagent 15 ml 198.8 EUR

Immuno HRP-AEC,ready to use IHC kit,anti-chicken IgY (H+L)

IH-8043-50 ImmunoBioscience each reagent 50ml 357.7 EUR

Immuno HRP-DAB,ready to use IHC kit,anti-chicken IgY (H+L)

IH-8053-15 ImmunoBioscience each reagent 15 ml 198.8 EUR

Immuno HRP-DAB,ready to use IHC kit,anti-chicken IgY (H+L)

IH-8053-50 ImmunoBioscience each reagent 50 ml 357.7 EUR

Immunoglobulin G (IgG) Monoclonal Antibody (Rat), HRP

4-MAA544Ra21-HRP Cloud-Clone
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Description: A Mouse monoclonal antibody against Rat Immunoglobulin G (IgG). This antibody is labeled with HRP.

Immunoglobulin E (IgE) Monoclonal Antibody (Pig), HRP

4-MAA545Po21-HRP Cloud-Clone
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Description: A Mouse monoclonal antibody against Pig Immunoglobulin E (IgE). This antibody is labeled with HRP.

Anti- Immunoglobulin A (IgA) Secretory -HRP Antibody

GWB-68E8A0 GenWay Biotech 1 ml Ask for price

Immunoglobulin G (IgG) Polyclonal Antibody (Dog), HRP

4-PAA544Ca01-HRP Cloud-Clone
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Description: A Rabbit polyclonal antibody against Dog Immunoglobulin G (IgG). This antibody is labeled with HRP.

Immunoglobulin G (IgG) Polyclonal Antibody (Cat), HRP

4-PAA544Fe01-HRP Cloud-Clone
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Description: A Rabbit polyclonal antibody against Cat Immunoglobulin G (IgG). This antibody is labeled with HRP.

Immunoglobulin G (IgG) Polyclonal Antibody (Pig), HRP

4-PAA544Po01-HRP Cloud-Clone
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Description: A Rabbit polyclonal antibody against Pig Immunoglobulin G (IgG). This antibody is labeled with HRP.

Immunoglobulin G (IgG) Polyclonal Antibody (Rat), HRP

4-PAA544Ra01-HRP Cloud-Clone
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Description: A Rabbit polyclonal antibody against Rat Immunoglobulin G (IgG). This antibody is labeled with HRP.

Immunoglobulin E (IgE) Polyclonal Antibody (Pig), HRP

4-PAA545Po01-HRP Cloud-Clone
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Description: A Rabbit polyclonal antibody against Pig Immunoglobulin E (IgE). This antibody is labeled with HRP.

Immunoglobulin G (IgG) Fab`2, Preadsorbed, Sheep -HRP

GWB-7D1B95 GenWay Biotech 0.5 mg Ask for price

Immunoglobulin G (IgG) Monoclonal Antibody (Goat), HRP

4-MAA544Cp25-HRP Cloud-Clone
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Description: A Mouse monoclonal antibody against Goat Immunoglobulin G (IgG). This antibody is labeled with HRP.

Immunoglobulin G (IgG) Polyclonal Antibody (Goat), HRP

4-PAA544Cp01-HRP Cloud-Clone
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Description: A Rabbit polyclonal antibody against Goat Immunoglobulin G (IgG). This antibody is labeled with HRP.

Immunoglobulin Heavy Constant Mu (IGHM) Antibody (HRP)

abx345748-100l Abbexa 100 µl 250 EUR

Immunoglobulin Heavy Constant Mu (IGHM) Antibody (HRP)

abx345748-50l Abbexa 50 µl 162.5 EUR

Immunoglobulin M (IgM) Monoclonal Antibody (Mouse), HRP

4-MAA543Mu21-HRP Cloud-Clone
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Description: A Rat monoclonal antibody against Mouse Immunoglobulin M (IgM). This antibody is labeled with HRP.

Immunoglobulin M (IgM) Monoclonal Antibody (Mouse), HRP

4-MAA543Mu29-HRP Cloud-Clone
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Description: A Rat monoclonal antibody against Mouse Immunoglobulin M (IgM). This antibody is labeled with HRP.

Immunoglobulin G (IgG) Monoclonal Antibody (Horse), HRP

4-MAA544Eq22-HRP Cloud-Clone
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Description: A Mouse monoclonal antibody against Horse Immunoglobulin G (IgG). This antibody is labeled with HRP.

Immunoglobulin G (IgG) Monoclonal Antibody (Human), HRP

4-MAA544Hu22-HRP Cloud-Clone
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Description: A Mouse monoclonal antibody against Human Immunoglobulin G (IgG). This antibody is labeled with HRP.

Immunoglobulin G (IgG) Monoclonal Antibody (Sheep), HRP

4-MAA544Ov22-HRP Cloud-Clone
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  • 20ul
Description: A Mouse monoclonal antibody against Sheep Immunoglobulin G (IgG). This antibody is labeled with HRP.

Immunoglobulin M (IgM) Polyclonal Antibody (Mouse), HRP

4-PAA543Mu01-HRP Cloud-Clone
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  • 100ul
  • 10ml
  • 1ml
  • 200ul
  • 20ul
Description: A Rabbit polyclonal antibody against Mouse Immunoglobulin M (IgM). This antibody is labeled with HRP.

Dual-color tracing the whole body in vivo fluorescence imaging of xenografts done using live imaging system. transplanted tumor frozen section prepared for histological analysis, to detect the presence of tumor cells invade blood vessels and cell fusion. dual-color images were able to distinguish between tumor cells red and green host cells. Results of this research suggest that the dual-color fluorescence-tracing glioma orthotopic implantation model may be convenient to detect the location of tumors, angiogenesis, cell fusion, and the tumor microenvironment.