An Analysis of the Practicalities of Multi-Color Nanoparticle Cellular Bar-Coding.

Many applications in biomedical research requires long-range identification and tracking of cells over time. In previous work we have shown that with sequential doses of a population of cells with nanoparticles of different emission wavelengths is possible to use a random number of nanoparticles loaded vesicles produced by cells as a barcode for individual cells in the population.

In this paper we develop a simple model to describe the amount of code that can be generated by using this sequential loading protocol. This methodology is validated in comparison with experiment and then used to predict the effects of various number of colors used to encode the cell and also to assess the effects of misread codes due to errors in the imaging cellular vesicles.

A Quick, Easy and Customizable Eight-color flow cytometric Method for Mobile Content Analysis of Bronchoalveolar Lavage Fluids in Mouse.

An Analysis of the Practicalities of Multi-Color Nanoparticle Cellular Bar-Coding.
An Analysis of the Practicalities of Multi-Color Nanoparticle Cellular Bar-Coding.

Cell composition of bronchoalveolar lavage fluid (BAL) is an important indicator of airway inflammation. It is generally determined by cytocentrifuging leukocytes on a slide, then staining, identify, and count them as eosinophils, neutrophils, macrophages, or lymphocytes according to morphological criteria under a light microscope, where it is not always easy to distinguish macrophages from the lymphocytes.

We describe here a single-step, easy to use, and easy-to-customize 8-color flow cytometric method to perform differential cell count and compare it with the number of morphology on stained cytospins. This method identifies BAL cells by one step immunolabeling simultaneous procedure using antibodies to identify T cells, B cells, neutrophils, eosinophils, and macrophages.

morphological analysis of flow-sorted cell subsets are used to validate this protocol. An important advantage of flow cytometry basic protocol is the ability to customize by adding antibodies to study the expression of receptors on the cell surface of leukocytes and identify subclasses of inflammatory cells as needed. © 2017 by John Wiley & Sons, Inc.

The advantage of dual-color fluorescence-tracing glioma orthotopic implantation model: Detecting the location of the tumor, angiogenesis, cell fusion and the tumor microenvironment.

Various organs of the body have different microenvironments with diverse biological characteristics that can influence tumor growth in themselves. However, the mechanisms underlying the interaction between tumor cells and the host is not currently well understood.

In this study, glioma model is the implantation of orthotopic fluorescence-tracing dual-color developed, in which the C6 cells rat glioma is labeled with a fluorescent dye red CM-Dil and glioma cells SU3 man stably express the protein fluorescence red, were inoculated into the right caudate nucleus of GMOs female C57BL / 6 nude mice revealed enhanced green fluorescent protein.

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beta-Actin Antibody - HRP

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Concanavalin A Lectin (ConA)-HRP conjugate

CONA15-HRP 0.5 ml
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50121-HRP 0.5 ml
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Rabbit Anti-Bird IgM-HRP Conjugate

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Monoclonal Anti-Phosphotyrosine (PY20) IgG-HRP conjugate

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Wheat germ agglutinin Lectin (WGA)-HRP conjugate

WGA15-HRP 0.5 ml
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60600-HRP 0.5 ml
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Rabbit Anti-Donkey IgG (H+L)-HRP conjugate

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Goat Anti-Human Plasma Fibrinogen IgG-HRP Conjugate

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Monoclonal Anti-Maltose binding protein (MBP)-HRP conjugate

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Goat Anti-Human plasminogen protein IgG, HRP conjugate

PLMN12-HRP 100 ul
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RCA15-HRP 0.5 ml
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Rabbit Anti-Human alpha-Tubulin IgG-HRP Conjugate

TUBA13-HRP 100 ul
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Goat Anti-Adenovirus type 2, hexon IgG-HRP conjugate

ADV11-HRP 0.5 ml
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Rabbit anti-Beta-Galactosidase (E. Coli) IgG-HRP conjugate

BGAL12-HRP 100 ul
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Rabbit Anti-human spleen ferrtin protein IgG, HRP conjugate

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Goat Anti-Hepatitis Surface Antigen (HBsAg) IgG-HRP Conjugate

HBA11-HRP 1 ml
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Mouse Monoclonal Anti-Hepatitis Surface Antigen (HBsAg)-HRP Conjugate

HBA12-HRP 1 ml
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Goat Anti-Poliomyelitis Virus 1-3 IgG-HRP Conjugate

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Goat Anti-Rubella virus (HPV77 strain) IgG-HRP conjugate

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Rabbit Anti-Bovine Serum Albumin (BSA) protein IgG-HRP Conjugate

ALBB14-HRP 0.5 ml
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Rabbit Anti-Human beta 2-microglobulin protein IgG, HRP conjugate

B2M11-HRP 100 ul
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Goat Anti-Human Alpha 1-Antitrypsin (A1AT) IgG, HRP conjugate

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Bovine Fetuin (Alpha-2 HS-Glycoprotein, AHSG, A2HS)-HRP Conjugate

FETB15-HRP 500 ul
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Goat Anti-Glutathione Transferase (GST, S. japonicum) IgG-HRP Conjugate

GST13-HRP 100 ul
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Goat Anti-Hemeagglutinin fusion epitope (HA-tag) IgG-HRP conjugate

HA12-HRP 100 ug
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Rabbit Anti-c-Myc epitope (fusion tag) IgG-HRP conjugate

MYC13-HRP 100 ul
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Goat Anti-Human Respiratory Syncytial Virus (RSV) IgG-HRP conjugate

RSV12-HRP 0.5 ml
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Monoclonal Anti-Vesicular Stomatitis Virus Glycoprotein (VSV)-IgG-HRP conjugate

VSV11-HRP 100 ul
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Goat Anti-human C-Reactive Protein (CRP)-HRP Conjugate, aff pure

CRP11-HRP 100 ug
EUR 408.00

Goat Anti-human Alpha 2-Macroglobulin (A2M) protein IgG, HRP conjugate

A2MG11-HRP 100 ul
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Goat Anti-human Kappa light chain (bound and free)-HRP conjugate

MKUL11-HRP 0.5ml
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Rabbit Anti-T7 tag (T7-tag, fusion tag) IgG-HRP Conjugate

T711-HRP 50 ul
EUR 408.00

Rabbit Anti-Vaccinia virus (VACV/VV/small pox) IgG-HRP conjugate

VACV11-HRP 0.5 ml
EUR 408.00

Mouse Monoclonal Anti-Human Beta Actin peptide (non-muscle) IgG-HRP Conjugate

ACTB12-HRP 100 ul
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Rabbit Anti-rat C-Reactive Protein (CRP) IgG-HRP Conjugate, aff pure

CRP16-HRP 100 ug
EUR 408.00

Goat Anti-rat C-Reactive Protein (CRP) IgG-HRP Conjugate, aff pure

CRP17-HRP 100 ug
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Goat Anti-Dog C-Reactive Protein (CRP) IgG-HRP Conjugate, aff pure

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Rabbit Anti-Chicken IgG/Y (ab')2 fragment specific IgG-HRP Conjugate

60445-HRP 0.5 ml
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Goat Anti-Green Fluorescent Proteins (GFP, A. victoria) protein IgG-HRP conjugate

GFP11-HRP 100 ug
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Monoclonal Anti-Green Fluorescent Proteins (GFP, A. victoria) protein IgG-HRP conjugate

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Monoclonal Anti-Rabbit IgG (Light-chain) IgG-HRP Conj (adsorbed, for immunoprecipitation/WB)

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Rabbit Anti-Human Alpha 1-Acid Glycoprotein (A1-AGP) protein IgG, HRP conjugate

A1AG11-HRP 100 ul
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Saccharomyces Cerevisiae (HCPs) IgG-HRP conjugate (100X)

760-160-HRP 1 ml
EUR 590.00

Mouse Monoclonal Anti-Goat IgG-HRP Conjugate (H+L, adsorbed with mouse, rat, rabbit, human, horse etc)

30226-HRP 0.5 ml
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Bovine IgG1-HRP conjugate (Serum Control, non-immune) purified

20001-G1-HRP 100 ug
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20001-G2-HRP 100 ug
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770-165-HRP 1 ml
EUR 590.00

Pichia Pastoris Host Cell Proteins (HCPs) IgG-HRP conjugate (100X)

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740-150-HRP 1 ml
EUR 590.00

Rabbit anti-Spodoptera frugiperda host cell Proteins (HCPs) IgG-HRP conjugate (100X)

730-145-HRP 1 ml
EUR 590.00

Rabbit Anti-Chinese Hamster Ovary Cell Host Cell Proteins (CHO-HCPs) IgG-HRP conjugate (100X)

800-140-HRP 1 ml
EUR 841.00

HRP 2 (HRP 2) Antibody

abx234012-100ug 100 ug
EUR 481.00
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Progesterone 3-HRP Conjugate Protein (HRP)

abx060852-1ml 1 ml
EUR 739.00
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Anti-Bovine Plasma Proteins

BPP04AF-HRP 100 ul
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Anti-CHO HCP

C0016AF-HRP 1 mg
EUR 4069.00
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Anti-E. coli HCP, Affinity Purified:HRP

AP117-HRP 1 mg
EUR 4069.00
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Peroxidase (72 U/mg)

HRP-01 100 mg
EUR 590.00

Peroxidase (250 U/mg)

HRP-02 100 mg
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Anti-NS/0 HCP

N9192AF-HRP 1 mg
EUR 4069.00
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Anti-CHO-CM HCP

M0016AF-HRP 1 mg
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Description: Anti-CHO-CM HCP by Cygnus Technologies is available in Europe via Gentaur.

Anti-SP2/0 HCP

SP110AF-HRP 1 ml
EUR 835.00
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Anti-Vero Cell HCP

VC807AF-HRP 1 mg
EUR 4069.00
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Benzodiazepine (HRP)

abx080001-1ml 1 ml
EUR 926.00
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HRP Antibody

20-abx134340
  • EUR 356.00
  • EUR 537.00
  • EUR 217.00
  • 100 ul
  • 200 ul
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HRP-HBsAg

C050101-10ml 10ml
EUR 2635.00

HRP-HBsAg

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EUR 438.00

HRP-SPA

C050102-10ml 10ml
EUR 1368.00

HRP-SPA

C050102-1ml 1ml
EUR 269.00

HRP-SPG

C050103-10ml 10ml
EUR 1368.00

HRP-SPG

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EUR 269.00

HRP-BSA

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EUR 1114.00

HRP-BSA

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EUR 227.00

HRP-CLE

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HRP-CLE

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EUR 438.00

HRP-SAL

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EUR 2635.00

HRP-SAL

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EUR 438.00

HRP-SA

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EUR 1368.00

HRP-SA

C050109-1ml 1ml
EUR 269.00

HRP-HSA

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EUR 1114.00

HRP-HSA

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EUR 227.00

HRP-OVA

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HRP-OVA

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HRP-CAP

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HRP-CAP

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HRP antibody

20C-CR7015GP 500 ul
EUR 80.00
Description: Goat polyclonal HRP antibody

HRP antibody

10R-1969 100 ul
EUR 381.00
Description: Mouse monoclonal HRP antibody

HRP antibody

10-3154 1 mg
EUR 208.00
Description: Mouse Monoclonal HRP antibody

HRP antibody

10R-P156a 1 mg
EUR 392.00
Description: Mouse monoclonal HRP antibody

HRP antibody

20R-3010 100 µg
EUR 647.00
Description: Rabbit polyclonal HRP antibody

Digoxin-HRP

65-ID15 50 units
EUR 398.00
Description: Conjugated Digoxin-HRP hapten

Methamphetamine-HRP

65-IM52 1 ml
EUR 444.00
Description: Conjugated Methamphetamine-HRP hapten

Phencyclidine-HRP

65-IP10 1 ml
EUR 440.00
Description: Conjugated Phencyclidine-HRP hapten

Progesterone-HRP

65-IP28 1 ml
EUR 480.00
Description: Conjugated Progesterone-HRP hapten

Triiodothyronine-HRP

65-IT35 50 units
EUR 381.00
Description: Conjugated Triiodothyronine-HRP hapten

Tobramycin-HRP

65-IT55 500 ug
EUR 500.00
Description: Conjugated Tobramycin-HRP Hapten

HRP Antibody

abx015753-100ul 100 ul
EUR 411.00
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Streptavidin (HRP)

abx670350-1mg 1 mg
EUR 398.00
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Streptavidin (HRP)

abx670354-1ml 1 ml
EUR 467.00
  • Shipped within 1 week.

DHEA-HRP

80-1056 500 ul
EUR 812.00
Description: Conjugated DHEA-HRP hapten

Nortestosterone-HRP

80-1078 500 ul
EUR 137.00
Description: 17a-19-Nortestosterone 3 Conjugate for use in immunoassays

MDMA-HRP

80-1091 500 ul
EUR 136.00
Description: MDMA n Conjugate for use in immunoassays

CLAD-HRP

80-1092 500 ul
EUR 248.00
Description: CLAD 3 Conjugate for use in immunoassays

Nitrophenyl-HRP

80-1093 500 ul
EUR 417.00
Description: 4-Nitrophenyl Conjugate for use in immunoassays

Acepromazine-HRP

80-1097 500 ul
EUR 809.00
Description: Acepromazine Conjugate for use in immunoassays

Albendazole-HRP

80-1098 500 ul
EUR 809.00
Description: Albendazole Conjugate for use in immunoassays

Albendazole-HRP

80-1099 500 ul
EUR 248.00
Description: Albendazole Conjugate for use in immunoassays

Amikacin-HRP

80-1100 500 ul
EUR 1207.00
Description: Amikacin Conjugate for use in immunoassays

Ampicillin-HRP

80-1102 500 ul
EUR 137.00
Description: Ampicillin Conjugate for use in immunoassays

Amphetamine-HRP

80-1104 500 ul
EUR 248.00
Description: Amphetamine m Conjugate for use in immunoassays

Androstanedione-HRP

80-1105 500 ul
EUR 809.00
Description: Androstanedione 3 Conjugate for use in immunoassays

Apramycin-HRP

80-1106 500 ul
EUR 137.00
Description: Apramycin Conjugate for use in immunoassays

Avilamycin-HRP

80-1107 500 ul
EUR 607.00
Description: Avilamycin Conjugate for use in immunoassays

Azaperone-HRP

80-1108 500 ul
EUR 417.00
Description: Azaperone Conjugate for use in immunoassays

Bacitracin-HRP

80-1109 500 ul
EUR 417.00
Description: Bacitracin Conjugate for use in immunoassays

Boldenone-HRP

80-1110 500 ul
EUR 137.00
Description: Boldenone 17 Conjugate for use in immunoassays

Buprenorphine-HRP

80-1111 500 ul
EUR 137.00
Description: Buprenorphine 3 Conjugate for use in immunoassays

Norbuprenorphine-HRP

80-1112 500 ul
EUR 417.00
Description: Norbuprenorphine 3 Conjugate for use in immunoassays

Carazolol-HRP

80-1113 500 ul
EUR 402.00
Description: Carazolol Conjugate for use in immunoassays

Carbadox-HRP

80-1114 500 ul
EUR 137.00
Description: Carbadox Conjugate for use in immunoassays

Carbamazepine-HRP

80-1115 500 ul
EUR 248.00
Description: Carbamazepine Conjugate for use in immunoassays

Ceftiofur-HRP

80-1116 500 ul
EUR 137.00
Description: Ceftiofur Conjugate for use in immunoassays

Cephalexin-HRP

80-1117 500 ul
EUR 414.00
Description: Cephalexin Conjugate for use in immunoassays

Chloramphenicol-HRP

80-1118 500 ul
EUR 417.00
Description: Chloramphenicol Conjugate for use in immunoassays

Chlorpromazine-HRP

80-1120 500 ul
EUR 417.00
Description: Chlorpromazine Conjugate for use in immunoassays

Cimaterol-HRP

80-1121 500 ul
EUR 137.00
Description: Cimaterol Conjugate for use in immunoassays

Clenbuterol-HRP

80-1122 500 ul
EUR 403.00
Description: Clenbuterol N Conjugate for use in immunoassays

Closantel-HRP

80-1123 500 ul
EUR 248.00
Description: Closantel Conjugate for use in immunoassays

CLAD-HRP

80-1125 500 ul
EUR 248.00
Description: CLAD 3 Conjugate for use in immunoassays

Cocaine-HRP

80-1127 500 ul
EUR 137.00
Description: Cocaine Conjugate for use in immunoassays

Benzoylecgonine-HRP

80-1128 500 ul
EUR 137.00
Description: Benzoylecgonine COOH Conjugate for use in immunoassays

Cortisone-HRP

80-1131 500 ul
EUR 412.00
Description: Cortisone Conjugate for use in immunoassays

Creatinine-HRP

80-1133 500 ul
EUR 133.00
Description: Creatinine Conjugate for use in immunoassays

cAMP-HRP

80-1134 500 ul
EUR 248.00
Description: cAMP Conjugate for use in immunoassays

Cyclopentobarbital-HRP

80-1135 500 ul
EUR 248.00
Description: Cyclopentobarbital  5 Conjugate for use in immunoassays

Dapsone-HRP

80-1136 500 ul
EUR 137.00
Description: Dapsone Conjugate for use in immunoassays

Decoquinate-HRP

80-1137 500 ul
EUR 137.00
Description: Decoquinate Conjugate for use in immunoassays

Dehydromethyltestosterone-HRP

80-1139 500 ul
EUR 417.00
Description: Dehydromethyltestosterone 3 Conjugate for use in immunoassays

Desipramine-HRP

80-1140 500 ul
EUR 137.00
Description: Desipramine Conjugate for use in immunoassays

Desmethyllnoproxen-HRP

80-1141 500 ul
EUR 414.00
Description: Desmethyllnoproxen Conjugate for use in immunoassays

Dual-color tracing the whole body in vivo fluorescence imaging of xenografts done using live imaging system. transplanted tumor frozen section prepared for histological analysis, to detect the presence of tumor cells invade blood vessels and cell fusion. dual-color images were able to distinguish between tumor cells red and green host cells. Results of this research suggest that the dual-color fluorescence-tracing glioma orthotopic implantation model may be convenient to detect the location of tumors, angiogenesis, cell fusion, and the tumor microenvironment.