Description: This product includes 60 ul of 1000 x PSA dye for more than 200 assays with the assay volume of 0.3 ml using 96-well plates or cuvettes.

Description: For quantitative determination of long-chain fatty acid uptake in whole cells and evaluation of effects of ligands or drugs on fatty acid transport. Key Features: Safe. Non-radioactive assay. Fast and Sensitive. Homogenous "add-and-read" assay. No wash, lysis, or staining steps needed. Simple and Convenient. Can be automated as a high-throughput assay for fatty acid transport and modulator screens in cells. Method: FL 488/523nm. Samples: Adipocytes and other fatty acid transporting cells. Species: All. Procedure: Assay takes 2 hrs. Kit size: 100 Tests.

Description: Fatty acid uptake is an important therapeutic target for the treatment of many human diseases such as obesity, type 2 diabete and hepatic steatosis.

Description: The Cereblon Binding Assay Kit (FP) is designed for testing and profiling of Cereblon (CRBN) inhibitors using Fluorescence Polarization. The assay is a competitive binding assay, based on the binding of fluorescently-labeled Thalidomide, a CRBN binder, to purified recombinant CRBN._x000D_ CRBN is the substrate recognition component of a DCX (DDB1-CUL4-X-box) E3 protein ligase complex that mediates the ubiquitination and subsequent proteasomal degradation of target proteins. CRBN is a direct protein target for the immunomodulatory and antiproliferative activities of thalidomide analogues such as lenalidomide and pomalidomide._x000D_ The Cereblon Binding Assay Kit (FP) comes in a convenient 96-well format, with enough purified CRBN, Cy5-labeled Thalidomide, and assay buffer for 100 enzyme reactions. In addition, the kit includes the CRBN inhibitor Pomalidomide for use as an inhibitor control._x000D_
The key to the CRBN Assay Kit is the fluorescently-labeled Thalidomide. Using this kit, only one simple step on a microtiter plate is required for CRBN reactions. The Cy5-labeled Thalidomide is incubated with a sample containing CRBN to produce a change in fluorescent polarization that can be measured using a fluorescence reader.